In his 1st at bat the former Heisman Trophy winner hit an opposite-field double off Erie SeaWolves right-hander Beau Burrows.
He was stranded at second as the next batter flied out to end the inning.
Tebow stepped to the plate right after the Western Division tied the game with three runs in the top of the fifth. He grounded out to shortstop.
His next at-bat came in the seventh inning, when he flied out while facing Richmond Flying Squirrels right-hander Dillon McNamara.
With the tying run on first in the bottom of the ninth, Tebow struck out after fouling off three pitches against Harrisburg Senators right-hander Derek Self.